ParkinsonCanadaV1, Main, Exploration, bibRecord, 001961

Behavioral effects of elevated expression of human equilibrative nucleoside transporter 1 in mice

Identifieur interne : 001961 ( Main/Exploration ); précédent : 001960; suivant : 001962

Behavioral effects of elevated expression of human equilibrative nucleoside transporter 1 in mice

Auteurs : Sara Kost [Canada] ; CHAO SUN [Canada] ; WEI XIONG [Canada] ; Kathryn Graham [Canada] ; Carol E. Cass [Canada] ; James D. Young [Canada] ; Benedict C. Albensi [Canada] ; Fiona E. Parkinson [Canada]

Source :

Behavioural brain research [ 0166-4328 ] ; 2011.

RBID : Pascal:11-0336432

Descripteurs français

Pascal (Inist)
- Comportement, Temps réel, Réaction chaîne polymérase, Récepteur adénosinique, Caféine, Ethanol, Homme, Souris, Animal, Psychotrope, Analeptique respiratoire, Stimulant SNC, Transporteur nucléoside ENT1.
Wicri :
- topic : Homme.

English descriptors

KwdEn :
- Adenosine receptor, Animal, Behavior, CNS stimulant, Caffeine, ENT1 nucleoside transporter, Ethanol, Human, Mouse, Polymerase chain reaction, Psychotropic, Real time, Respiratory analeptic.

Abstract

Adenosine concentrations are regulated by purinergic enzymes and nucleoside transporters. Transgenic mice with neuronal expression of human equilibrative nucleoside transporter 1 (hENT1) have been generated (Parkinson et al., 2009 [7]). The present study tested the hypothesis that mice homozygous and heterozygous for the transgene exhibit differences in hENT1 mRNA and protein expression, and in behavioral responses to caffeine and ethanol, two drugs with adenosine-dependent actions. Real time polymerase chain reaction (PCR) was used to identify mice heterozygous and homozygous for the transgene. Gene expression, determined by real time PCR of cDNA reverse transcribed from cerebral cortex RNA, was 3.8-fold greater in homozygous mice. Protein abundance, determined by radioligand binding assays using 0.14nM [³H]S-(4-nitrobenzyl)-6-thioinosine ([³H]NBTI), was up to 84% greater in cortex synaptosome membranes from homozygous than from heterozygous mice. In western blots with an antibody specific for hENT1, a protein of approximately 40 kDa was strongly labelled in cortex samples from homozygous mice, weakly labelled in samples from heterozygous mice and absent from samples from wild type mice. In behavioral assays, transgenic mice showed a greater response to ethanol and a reduced response to caffeine than wild type littermates; however, no significant differences between heterozygous and homozygous mice were detected. These data indicate that the difference in ENT1 function between wild type and heterozygous mice was greater than that between heterozygous and homozygous mice. Therefore, either heterozygous or homozygous hENT1 transgenic mice can be used in studies of ENT1 regulation of adenosine levels and adenosine dependent behaviors.

Affiliations:

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Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenosine receptor</term>
<term>Animal</term>
<term>Behavior</term>
<term>CNS stimulant</term>
<term>Caffeine</term>
<term>ENT1 nucleoside transporter</term>
<term>Ethanol</term>
<term>Human</term>
<term>Mouse</term>
<term>Polymerase chain reaction</term>
<term>Psychotropic</term>
<term>Real time</term>
<term>Respiratory analeptic</term>
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<keywords scheme="Pascal" xml:lang="fr"><term>Comportement</term>
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<term>Récepteur adénosinique</term>
<term>Caféine</term>
<term>Ethanol</term>
<term>Homme</term>
<term>Souris</term>
<term>Animal</term>
<term>Psychotrope</term>
<term>Analeptique respiratoire</term>
<term>Stimulant SNC</term>
<term>Transporteur nucléoside ENT1</term>
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<front><div type="abstract" xml:lang="en">Adenosine concentrations are regulated by purinergic enzymes and nucleoside transporters. Transgenic mice with neuronal expression of human equilibrative nucleoside transporter 1 (hENT1) have been generated (Parkinson et al., 2009 [7]). The present study tested the hypothesis that mice homozygous and heterozygous for the transgene exhibit differences in hENT1 mRNA and protein expression, and in behavioral responses to caffeine and ethanol, two drugs with adenosine-dependent actions. Real time polymerase chain reaction (PCR) was used to identify mice heterozygous and homozygous for the transgene. Gene expression, determined by real time PCR of cDNA reverse transcribed from cerebral cortex RNA, was 3.8-fold greater in homozygous mice. Protein abundance, determined by radioligand binding assays using 0.14nM [<sup>3</sup>
H]S-(4-nitrobenzyl)-6-thioinosine ([<sup>3</sup>
H]NBTI), was up to 84% greater in cortex synaptosome membranes from homozygous than from heterozygous mice. In western blots with an antibody specific for hENT1, a protein of approximately 40 kDa was strongly labelled in cortex samples from homozygous mice, weakly labelled in samples from heterozygous mice and absent from samples from wild type mice. In behavioral assays, transgenic mice showed a greater response to ethanol and a reduced response to caffeine than wild type littermates; however, no significant differences between heterozygous and homozygous mice were detected. These data indicate that the difference in ENT1 function between wild type and heterozygous mice was greater than that between heterozygous and homozygous mice. Therefore, either heterozygous or homozygous hENT1 transgenic mice can be used in studies of ENT1 regulation of adenosine levels and adenosine dependent behaviors.</div>
</front>
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<name sortKey="Albensi, Benedict C" sort="Albensi, Benedict C" uniqKey="Albensi B" first="Benedict C." last="Albensi">Benedict C. Albensi</name>
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<name sortKey="Cass, Carol E" sort="Cass, Carol E" uniqKey="Cass C" first="Carol E." last="Cass">Carol E. Cass</name>
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<name sortKey="Parkinson, Fiona E" sort="Parkinson, Fiona E" uniqKey="Parkinson F" first="Fiona E." last="Parkinson">Fiona E. Parkinson</name>
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	La maladie de Parkinson au Canada (serveur d'exploration)
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La maladie de Parkinson au Canada (serveur d'exploration)

Behavioral effects of elevated expression of human equilibrative nucleoside transporter 1 in mice

Behavioral effects of elevated expression of human equilibrative nucleoside transporter 1 in mice

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri